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The purpose of this study was to determine the effect of a commercially available alcohol-based hand-rub (Alcohol Plus) on the time to sterility and the number of microorganisms in hard-surface model environments and compare these effects with those of a commercially available isopropyl alcohol solution (IPA) and a sodium hypochlorite solution. A total of 175 model surface sectors (9×9×1.5 cm) were inoculated with a 1:10-dilution of a standardized suspension containing known concentrations of a bacterium or a yeast. The following concentrations were tested: a sodium hypochlorite concentration of 0.1% (HT-0.1); 1% (HT-1); 2.5% (HT-2.5); 5% (HT-5); IPA; or Alcohol Plus. Sterility was determined by visual inspection after inoculation and after a period of storage at 22°C. Microbial regrowth data were generated by measurement of the number of viable microorganisms after storing the sectors at 22°C for 10 days. Solutions of 2.5% and 5% HT-2.5 and HT-5 both increased the number of surviving microorganisms on model surfaces. However, this effect was not observed with 2.5% and 5% HT-0.1 and did not translate to a significant increase in the time to sterility (p>0.05). The concentrations of 2.5% and 5% HT-2.5 and HT-5 were significantly more efficacious than the IPA and Alcohol Plus solutions (p