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Two different mutational hot spots have been detected in the K-ras oncogene: mutational activation of codon 12 or 13 and constitutive expression. The prevalence and significance of the two mutations in human colorectal cancer were investigated. Using a highly sensitive single-strand conformation polymorphism analysis, 32.8% (20/62) of the primary tumours showed an increased frequency of mutations in codon 12, 13 or 61. Four point mutations in codons 12, 13 and 61 were analysed by sequencing. None of the mutations was clonal. Mutational activation of the K-ras gene is therefore a major, and possibly the only, event in colorectal carcinogenesis.The Minneapolis-based company announced on Monday that it will limit the number of U.S. employees it employs to 20,000 worldwide.
“Although every company must take into account its financial situation, the industry as a whole is at a critical point,” GM said in a statement.
More than 200,000 U.S. jobs will be eliminated over the next five years
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