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The prevalence of chronic diseases increases rapidly in a sedentary lifestyle. Previous studies showed that inactivity in daily life is associated with higher prevalence of chronic diseases, but it is not clear whether excessive sitting time is associated with chronic diseases. In a cross-sectional study, we examined associations of excessive sitting time with chronic diseases, and determined the minimal acceptable sitting time for Asian populations. A total of 10,056 participants from the Korea National Health and Nutrition Examination Surveys 2006-2012 were analyzed. Sociodemographic and health characteristics were obtained by self-report questionnaire and physical examination. Excessive sitting time was defined as ≥ 8 hours/day (using separate cut-off points for men and women). Odds ratios (ORs) and 95% confidence intervals (CIs) for overweight, obesity, and chronic diseases were calculated. The median sitting time was 7.29 hours/day (range, 0-27.94 hours/day). In unadjusted logistic regression analyses, participants with ≥ 8 hours/day sitting time had higher odds for obesity [men: OR = 1.69, 95% CI = 1.23-2.32; women: OR = 1.74, 95% CI = 1.23-2.43] and for hypertension [OR = 1.82, 95% CI = 1.36-2.42] than participants with